Saline solution is used for the preparation of culture suspension or serial dilution in microbiology. Saline is 0.9 % sodium chloride solution. Culture suspensions are prepared in saline solution because 0.9 % sodium chloride solution is isotonic in nature. In isotonic solution the concentration of solutes remains the same both inside and outside of the microbial cell and cells remains at their usual osmotic pressure. But water is hypotonic in nature in which the concentration of solute is greater inside the cell than outside and due to this water try to enter inside the bacterial cell. The osmotic pressure develop on the bacterial cell which can damage the bacterial cells during serial dilution. That's why serial dilution is prepared in 0.9% saline solution.
* Please Don't Spam Here. All the Comments are Reviewed by Admin.
