Friday, June 17, 2016

Growth Promotion Test of media

The purpose of the Growth Promotion Test is to determine the suitability of culture media. The medium is challenged with a small number of microorganisms to assure the nutritive properties. The test is described in USP Chapter <61>: Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests, USP Chapter <62>: Microbiological Examination of Nonsterile Products: Tests for Specified Substances and USP Chapter <71>: Sterility Tests.

We perform growth promotion testing (GPT) on solid media and broths according to Ph Eur and USP. The purpose of We perform growth promotion testing (GPT) on solid media and broths according to Ph Eur and USP. The purpose of the test is to ensure the quality and functionality of the media before using it for analysis. GPT should be performed on solid media and broths but not for dilution solutions and rinsing fluids.

GPT is performed by inoculating the product with ≤ 100 CFU of microorganisms, defined by the pharmacopoeia.  The test can be quantitative, at which 50 – 200% of the inoculated microorganisms should be recovered after incubation of the media, or it can be qualitative where the requirement is “growth” or “no growth”. GPT is described in Ph Eur 2.6.12 / USP (“Microbial Enumeration Test”), in Ph Eur 2.6.13 / USP (“Test for Specified Microorganisms”) and Ph Eur 2.6.1 / USP (“Sterility Test”)test is to ensure the quality and functionality of the media before using it for analysis. GPT should be performed on solid media and broths but not for dilution solutions and rinsing fluids.
GPT is performed by inoculating the product with ≤ 100 CFU of microorganisms, defined by the pharmacopoeia.  The test can be quantitative, at which 50 – 200% of the inoculated microorganisms should be recovered after incubation of the media, or it can be qualitative where the requirement is “growth” or “no growth”. GPT is described in Ph Eur 2.6.12 / USP (“Microbial Enumeration Test”), in Ph Eur 2.6.13 / USP (“Test for Specified Microorganisms”) and Ph Eur 2.6.1 / USP (“Sterility Test”)


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