The volume of mobile phase required to elute an un retained compound from column which is equal to column volume.
It is very much less possibility if the any impurity elutes before dead volume time, some specialized note shall be required during method development or method validation.
Otherwise method not reliable for evaluation if this impurity.
It is very much less possibility if the any impurity elutes before dead volume time, some specialized note shall be required during method development or method validation.
Otherwise method not reliable for evaluation if this impurity.
